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458can be improved in our institute by including the anterior portion of the Apex-Mid and Mid regions in the 12-core biopsy or 16-core biopsy, such that a 4-core biopsy of the anterior portion is included.During my 40 years at Juntendo University, I served three chief professors and two directors (Figure 9). Thanks to these doctors, I was able to carry out a very fulfilling and happy medical prac-tice. We would like to express our sincere gratitude to all the bosses and staff who have been involved in reaching retirement age.AcknowledgmentsNot applicable.Not applicable. 1) Wang MC, Valenzuela LA, Murphy GP, Chu TM, Purifi-cation of a human prostate specific antigen. invest Urol, 1979; 17: 159-163. 2) Holm HH, Gammelgaard J: Ultrasonically guided precise needle placement in the prostate and the seminal vesi-cles. J Urol, 1981; 125: 385-387. 3) Sakamoto Y, Fukaya K, Haraoka M, et al: Analysis of prostate cancer localization toward improved diag-nostic accuracy of transperineal prostate biopsy. Pros-tate Int, 2014; 2: 114-120.YS contributed to drafting the manuscript, figures and tables.The author declares that there are no conflicts of interest.FundingAuthor contributionsConflicts of interest statementReferences

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