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-Basic research (neutrophil phenotype and ATP)-Basic research includes neutrophil phenotype studies during invasion (e.g., during sepsis) and studies targeting ATP. The former includes the dynamics of aged neutrophils, ICAM-1 positive neutrophils and NETs, and identification of low- density neutrophil subsets. In the research on neutrophil ATP, we conducted studies on fluores-cence imaging of ATP on the surface of neutrophils and in mitochondria, and neutrophil signaling trans-duction (Figure 4) (6). Stimulation of the formyl peptide receptor was previously found to cause ATP release from PMNs through maxi-anion chan-nels and PANX1 hemichannels. To confirm this finding, the median fluorescence intensity (MFI) of PMAP-1 on the plasma membrane of healthy control (HC) PMNs was quantified by flow cytom-etry after stimulation with the indicated concentra-tions of fMLP (Figure 5a). The MFI of PMAP-1 following fMLP stimulation increased in a dose-de-pendent manner. CD11b expression on the plasma while registry studies in pediatric emergencies included prognostic comparisons of pediatric severe trauma, and epidemiological studies of critically ill pediatric patients. Clinical research in the field of intensive care includes systemic review on hyper-osmotic infusion in patients with severe head trauma and the development of a prognostic model for patients with cardiopulmonary arrest (4).-Disaster medicine and pre-hospital emergency medicine-Studies in the field of disaster medicine and pre- hospital emergency medicine involves those on the establishment of business continuing plans (BCPs) for disasters. Other studies include research on the effectiveness of Rapid Response Car (5), the estab-lishment of an emergency medical care system in large theme parks, and research on the effective-ness of electronic triage during disasters, which was adopted by CREST.Clinical researchStudies on ER science Triage by rapid lactate measurement Dizziness and heart rate variabilityRegistry studies in pediatric emergencies Prognostic comparison of pediatric severe trauma Epidemiological studies of critical ill pediatric pateientIntensive care field Systemic review on hyperosmotic infusion in severe head injury Prognostic model for patients with cardiopulmonary arrestRole of complement system in patients with multiple organ failure (KAKENHI Grant number 19H03764)Disaster and Prehospital researchBCPs for disasters Studies on securing intensive care staff during a pandemic Studies on water supply in the earthquake Effectiveness rapid response car Establishment of an emergency medical care system in large theme park Effectiveness of electronic triage during disasters (CREST)Basic researchNeutrophil phenotype studies in sepsis Dynamic of aged neutrophils ICAM-1 positive neutrophils and NETs Identification of low-density neutrophilATP targeting studies Fluorescence imaging of ATP on the surface of neutrophils and in mitochondria Neutrophil signaling transductionJoint research courseEmergency AI Color Image Information Standardization CourseTable 3　Our various research areas327