68-4

28/136

the relationship between CB function and glucocor-ticoids remains unclear. We investigated whether Rab8 interacts the mechanism of IOP elevation by glucocorticoids stimulation. Light microscopy did not show any morphological changes in the morphology and protein expression of cultured cynomolgus monkey non-pigmented ciliary epithe-lial cells with different concentrations of DEX(data not shown). Western blotting of epithelial cell lysates showed a tendency for the addition of DEX to decrease the expression of Rab8 protein and this effect seemed dose-dependent, though this is not significant(Figure 3A). The experiments repeated Figure 2　 Immunohistochemistry of normal cynomolgus monkey ciliary body, and immunofluorescence staining of cultured monkey ciliary body cellsParaffin sections of cynomolgus monkey ciliary body (CB) were labeled with antibodies specific to Rab8 (A-C), ezrin (A), radixin (B), and moesin (C). Monkey CB cells were stained with Rab8 and ezrin (D). Co-expression of Rab8 and the ezrin/radixin/moesin (ERM) family can be seen at the ciliary non-pigmented epithelium cells (arrow).348twice and the results were similar. In addition, when cells were cultured in the same way and timolol maleate or acetazolamide was added, no changes were observed in the expression of Rab8(-Figure 3B, 3C).Proteomic analyses of the CB of cynomolgus monkeys detected many proteins, some of which are involved in protein transport and secretion. In the CB, the difference of protein characteristics was found between ciliary epithelium and stroma. Structure proteins were major component in stroma, Discussion