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(2)Experimental studies on relationship between the effects of the donor’s fasting and graft viability after liver transplantation434Figure 4　The figure is that the upper hepatic vena cava has been sutured with 7-0 proline.Figure 5　The figure shows reconstructions of the portal vein and the inferior vena cava by Kamada’s cuff method. The bile duct is reconstructed by end-to-end anastomosis with a stent tube.We conducted a study called “Experimental studies on relationship between the effects of the donor’s fasting and graft viability after liver trans-plantation”1）.Using Wister male rats, the donors were classi-fied into 6 groups with fasting times of 0 hour, 48 hours, and 72 hours, and donor liver cold ischemic times of 0 hour and 6 hours. That is, the fasting time and cold ischemic time were 0 hour and 1 hour in group 1, 0 hour and 6 hours in group 2, 48 hours and 1 hour in group 3, 48 hours and 6 hours in group 4, 72 hours and 1 hour in group 5, and 72 hours and 6 hours in group 6, respectively. Euro-Collins solution was used as the preservation solution, but it is generally said that the Euro-Col-lins solution can preserve the donor liver for up to 6 hours. The measurement items were bile excre-tion 3 hours after liver transplantation, survival rate, liver tissue ATP, ADP, adenylate energy charge during liver cold preservation, liver weight, and liver tissue blood flow after liver transplanta-tion. Histological examinations were also performed.Bile excretion 3 hours after liver transplantation was significantly decreased in groups 4 and 5, and no bile excretion was observed in group 6, showing primary graft nonfunction (Figure 6).The survival rate after liver transplantation was 80% or more in groups 1, 2 and 3, but all died within 24 hours in groups 4 and 6 and all died within 28 days in group 5, no long term survival was obtained (Figure 7).Liver tissue ATP during hepatic cold preserva-tion was significantly lower in the 72-hour fasting group (Figure 8).Liver tissue blood flow after liver transplantation was measured by the hydrogen gas clearance method by developing a coil-shaped electrode. Liver tissue blood flow after liver transplantation was significantly reduced in group 5 and markedly decreased in groups 4 and 6.Electron micrographs of liver tissue showed marked degeneration of sinusoidal endothelial cells and microvilli fragmentation of the Disse cavity in Groups 4 and 6.Figure 6　Bile excretion 3 hours after liver transplantation was significantly decreased in groups 4 and 5, and no bile excretion was observed in group 6, showing primary graft nonfunction.