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Takahiro NIIZUMA1), Kaoru OBINATA1), Keiji KINOSHITA2), Toshiaki SHIMIZU3)2)Department of Pediatrics, Koshigaya Municipal Hospital, Saitama, Japan3)Department of Pediatrics, Juntendo University Faculty of Medicine, Tokyo, JapanCorresponding author: Takahiro NiizumaDepartment of Pediatrics, Juntendo University Urayasu Hospital2-1-1 Tomioka, Urayasu, Chiba 279-0021, JapanTEL: +81-47-353-3111　FAX: +81-47-353-0526　E-mail: zumani@amber.plala.or.jp〔Received　Jan. 12, 2021〕〔Accepted　May. 26, 2021〕J-STAGE Advance published date: Aug. 11, 2021Copyright © 2021 The Juntendo Medical Society. This is an open access article distributed under the terms of Creative Commons Attribution License (CC BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original source is properly credited. doi: 10.14789/jmj.JMJ21-OA01Objective: A hospital clerk developed whooping cough and its infectious condition throughout the work place was investigated to clarify the spread of pertussis infection using highly sensitive Loop-mediated isothermal amplification (LAMP).Materials and Methods: LAMP for pertussis was performed against all employees in the personnel division of general hospital, then respiratory symptoms were noted in 12 (57%) of 21 clerical employees.Results: LAMP for pertussis was positive in 7 (58.3%) of the 12 employees. The judgment was indeterminate in the first test in one employee, but it became negative later. An asymptomatic LAMP-negative employee subsequently developed cough and turned to positive in the second LAMP test 20 days after the first test. Cough was not noted even in positive cases in the third LAMP test 35 days after second test, or thereafter, and conversion to LAMP-negative took 76 days on average. No outbreak of whooping cough occurred in any of the other departments in the hospital throughout the course, and no transmission of infection occurred. LAMP methods were useful for grasp of the situation of asymptomatic persons as well as symptomatic patients.Conclusions: It is difficult to diagnose whooping cough based on cough symptoms alone. The LAMP test was useful for understanding the spread of pertussis infection. To prevent outbreak of whooping cough in adults, periodic administration of DTap vaccine is desired.Key words: hospital office, pertussis, LAMPJuntendo Medical Journal2021. 67(4), 355-359Original ArticlesPertussis Transmission in a Hospital Office that was Confirmed on the IntroductionPertussis has recently been attracting attention as a cause of prolonged cough in adults. Based on a serological survey of health care workers, employees are latently exposed to and infected with pertussis1). For diagnosis, in addition to conventional Bordetella pertussis culture and sero-logical tests, loop-mediated isothermal amplifica-tion (LAMP) as a Bordetella pertussis gene test became covered by national health insurance in October 2016 in Japan and is now widely used for 1)Department of Pediatrics, Juntendo University Urayasu Hospital, Chiba, Japandiagnosis in routine medical practice. In this study, a hospital clerk developed whooping cough and its spread in the work place was clarified using the LAMP method. LAMP methods were useful for grasp of the situation of asymptomatic persons as well as symptomatic patients.Case 1 (index case) aged 24 years old female, who developed a cough on September 12, 20XX without certain sick contact. Her working place was administrative department in general hospital. 355Basis of Loop-mediated Isothermal AmplificationMaterials